Solution Dilution Calculator (2024)

Proteinase K - Powder (20 mg)

Molecular Biology Grade: Liquid or White lyophilized powder Ultrapure Grade: White lyophilized powderStorage: -20°C

AllTaq PCR Core Kit (250 U)

50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water

T4 DNA Ligase (Rapid)

240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer

StableScript Reverse Transcriptase (250 U)

250 U of StableScript Reverse Transcriptase (0.05 mL at 5000 U/mL) and 4x StableScript Reaction Buffer (1 x 1.5 mL)

End-Repair Mix (low concentration)

For 200 low-concentration DNA reactions. End-Repair Mix 1X (0.20 mL), 10X End-Repair Buffer (1 x 1.5 mL) and 1 mM dNTP solution (0.5 mL).

Taq DNA Polymerase (250 U)

250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2

TaqNova Polymerase UCP (1000 U)

Ultra-Clean Production TaqNova DNA Polymerase.5 U/µL concentration with reaction buffer of 10x TaqNova DNA-free Reaction Buffer, and 50 mM MgCl2Storage buffer: 20 mM Tris-HCl (pH 8.0 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% (v/v) Nonident P40; 0.5% (v/v) Tween 20; 50% (v/v) glycerolStorage temperature: -20°C

TaqNova HS DNA Polymerase (500 U)

TaqNova HS DNA Polymerase, 10x TaqNova HS Reaction Buffer, 50 mM MgCl2Storage temperature: -20°C

RNase A (17,500 U)

2.5 ml (100 mg/ml; 7000 units/ml, solution)Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.

HotStarTaq DNA Polymerase (250 U)

250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2

Omniscript RT Kit (50)

For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water

RNase-Free DNase Set (50)

1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps

QIAGEN Protease (7.5 AU)

7.5 Anson units per vial (lyophilized)

QIAGEN Proteinase K (2 ml)

2 ml (>600 mAU/ml, solution)

Solution Dilution Calculator (2024)

FAQs

How do you calculate how much to dilute a solution? ›

To make a fixed amount of a dilute solution from a stock solution, you can use the formula: C1V1 = C2V2 where: V1 = Volume of stock solution needed to make the new solution. C1 = Concentration of stock solution.

How do you calculate value dilution? ›

The dilution is the percentage that is left to you (or in general all existing founders/officers/investors) after the new investment. If you own 100% of the company pre-money, the pre-money valuation is $x, and the post-money is $x+y, dilution would be x / (x+y).

How do you do a 5% dilution? ›

For example, a 1:5 dilution (verbalize as "1 to 5" dilution) entails combining 1 unit volume of diluent (the material to be diluted) + 4 unit volumes of the solvent medium (hence, 1 + 4 = 5 = dilution factor).

How to calculate how much stock solution is needed? ›

Stock Solution Dilutions
  1. C1 x V1 = C2 x V2
  2. C1 = stock concentration (beginning concentration)
  3. V1 = volume of stock required to prepare new solution.
  4. C2= concentration of new or working solution (desired concentration)
  5. V2= volume of new solution desired.
  6. Example:
  7. X = 160 mL of 5% stock solution diluted to 400 mL.

How do you calculate fully diluted? ›

To calculate fully diluted shares, add the number of common shares the company would issue upon the exercise or conversion of all the potentially dilutive securities mentioned above to the current number of outstanding common shares.

How do you calculate how much solution you need? ›

Since the density of water is 1 g/ml, the formula to calculate the amount of solute that must be mixed for a weight percent solution is: grams of solute = (wt% solution) x (ml of water) ÷ (100 – wt% solution)

What is the equation used for dilution calculations? ›

To dilute a stock solution, the following dilution equation is used: M1 V1 = M2 V2. M1 and V1 are the molarity and volume of the concentrated stock solution, and M2 and V2 are the molarity and volume of the diluted solution you want to make.

What is the formula for dilution ratio? ›

If given the ounces per gallon, calculate the dilution rate by dividing the concentrate ounce per gallon into 128. For example, if a product dilutes at 4-ounces per gallon, the calculation would be: 128 ÷ 4 = 32, or a dilution rate of 1:32.

How do you calculate the dilution of a sample? ›

Calculating a Dilution: the Dilution Formula

= concentration in molarity (mol/L or M) of the concentrated solution we start with. = volume (mL or L) of concentration solution we start with. = concentration in molarity (mol/L or M) of the diluted solution we end with.

What is the simple dilution method? ›

Simple Dilution Method

To obtain the desired concentration, a simple dilution is one in which a unit volume of a liquid material of interest is blended with an adequate volume of a solvent liquid. The total number of unit volumes in which the material will be dissolved is the dilution factor.

How do I calculate dilution factor? ›

The formula for dilution factor (or DF for short) is as follows: DF = (final volume of cells + stain)/(initial volume of cells). For example, If you mix your sample 1:1 with AO/PI, you'll need to add 20 uL AO/PI to 20 uL cells, for a total of 40 uL.

What is the formula for dilution calculator? ›

Dilute Solution of Known Molarity

The calculator uses the formula M1V1 = M2V2 where "1" represents the concentrated conditions (i.e., stock solution molarity and volume) and "2" represents the diluted conditions (i.e., desired volume and molarity).

How do you calculate stock dilution? ›

A basic formula for calculating equity dilution is to divide a current shareholder's total number of existing shares by the sum of the total number of outstanding shares + the total number of new shares, as shown in the example above.

How to dilute a solution to a specific concentration? ›

Measure the volume V1 of the solution with concentration C1. Then, add enough diluting liquid (water, etc.) to make a total volume V2. This new solution will have your desired concentration (C2).

How do you calculate how much water to dilute? ›

You normally use the formula C1V1 = C2V2 to calculate dilutions: C1 is the concentrated starting stock. V1 is the volume of starting stock required. C2 is the desired stock concentration.

What is the formula for dilute solution concentration? ›

Dilute Solution of Known Molarity

The calculator uses the formula M1V1 = M2V2 where "1" represents the concentrated conditions (i.e., stock solution molarity and volume) and "2" represents the diluted conditions (i.e., desired volume and molarity).

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