Why is an Accurate Pipette so Important? (2024)

FAQs

Why is an Accurate Pipette so Important? ›

Reproducible Experiments: An accurate pipette delivers precise volumes of reagents, samples, or solutions, allowing consistent replication of experiments for reliable scientific findings.

It's crucial to select a pipette that can accommodate the volume range required for your experiments. Additionally, ensure that the pipette offers the level of accuracy needed for your specific applications. High-precision experiments demand pipettes with tighter tolerances to ensure reliable and reproducible results.

Pipettes provide a clean and hygienic environment to aspirate, transport and dispense liquid samples. The purpose-built tools help to maintain quality assurance in the laboratory and ensure the most reliable and accurate results possible.

Accuracy is the ability of a pipette to give a response close to a true or nominal volume as indicated by the volume setting.

The volumetric pipette remains the world's most accurate. The article Good Pipetting Technique – Simple Ways to Minimise Errors discusses in more detail the ways in which you can achieve consistent pipetting results.

Reproducible Experiments: An accurate pipette delivers precise volumes of reagents, samples, or solutions, allowing consistent replication of experiments for reliable scientific findings.

Its advised to use you same Pipette through out the single experiment because: →Pipette requires cleaning after every use to prevent contamination. and to ensure delivery of the right amount of liquid next time when we are using.

Answer and Explanation:

Yes, it is possible when the volumetric pipette has some manufacturer defects in measurement or it measures the wrong volume. Accuracy is a parameter which measures the closeness of the observed value to the true value, since the pipette has a defect in measurement it will have low accuracy.

Routine testing of pipettes at regular intervals or even before each experiment is a recommended practice that can help confirm that the pipette delivers accurate results.

Environmental Conditions: Temperature, humidity, and air pressure can impact the accuracy of pipettes. It's important to work in a controlled environment and allow pipettes to equilibrate to room temperature before use.

Why is a pipette more accurate than a burette? ›

Another difference between Burette and Pipette is that the Burettes are larger than pipettes with higher capacity of liquid to dispense. That's why the accuracy of a burette in releasing small volumes is lesser than a pipette.

Graduated pipettes are considered to be more precise than the Pasteur pipette. They have tolerances that range from ±0.6% to ±0.4% of the nominal volume when measured at 20 °C (68 °F). Graduated pipettes are manufactured according to ISO specifications for accuracy and the arrangement of the graduations.

They recommend selecting a pipette with a nominal (maximum) volume as close as possible to the desired volume to transfer. Also, regardless of the required volume, physical properties of the liquid directly impact precision and accuracy.

Choose a pipette tip size based on the transfer capacity of the pipette with which it will be used and the amount of liquid being transferred. A large-capacity pipette and tip would not effectively transfer minute sample volumes.

If you keep using the same tip, you might contaminate other samples, as well as your reagents. In many laboratory workflows, you will need to change tips so often, that a micropipette has a specific ejector button for this.

Pipettes are typically used to transfer tiny amounts of liquid or to measure and dispense liquid in millilitres (mL) units. Micropipettes are more precise and convenient for measurements of less than 1 mL.