Posted January 9, 2024
Answer
The procedure for one type of ten-fold serial dilution is described in the steps below.
- Arrange the sterilized glass test tubes in a rack and mark each tube to show the level of dilution after performing the ten-fold serial dilution.
- Use a micropipette to add 900 mL of the diluent into all the labeled sterile tubes.
- Using a micropipette, transfer 100 mL of the test solution to the first tube and mix thoroughly. This marks the first ten-fold dilution.
- Use a fresh sterile tip on the micropipette to create a second ten-fold dilution
- Continue this sequence of ten-fold dilutions until reaching the last tube in the series.
These same steps can be followed for other experiments using different volumes, and diluents.
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