The Evolution of Respiratory Cryptosporidiosis: Evidence for Transmission by Inhalation (2024)

History

More than 20 Cryptosporidium species infect birds, reptiles, fish, and mammals, including humans (26,–28). Cryptosporidium was first named and reported in 1907, after its discovery in the stomachs of mice (29). After its original identification in animals, the first human Cryptosporidium infections were not reported until 1976 (30, 31). By 1980, only seven cases of human cryptosporidiosis had been confirmed (32,–36); five involved immunocompromised patients, and three were fatal. With the exception of one hospital-based survey in Australia (37), cryptosporidiosis in humans remained largely unknown as a primary cause of acute diarrheal disease until the emergence of the global AIDS pandemic, when Cryptosporidium infection became one of the first defining entities of AIDS, before the discovery of the etiologic virus (38).

Life Cycle and Infectivity

Cryptosporidium spp. propagate via a direct life cycle involving a single host. Oocysts containing four naked infectious sporozoites are shed in feces and ingested by a new host, whereby both asexual and sexual stages develop in the intestinal epithelium, leading to the release of new oocysts (39). Cryptosporidium oocysts are also capable of autoinfection, a unique ability of this parasite that allows it to sporulate and persist within the same host indefinitely (40). Oocysts may be encountered in contaminated water or food, on environmental surfaces, via fomites, or directly when shed by infected animals or people (41).

Cryptosporidium spp. are highly infectious; in human volunteer studies, as few as 10 Cryptosporidium hominis oocysts produced disease in healthy adults (42). A quantitative risk assessment has estimated that ingestion of a single oocyst of the C. parvum IOWA isolate will result in clinical disease in 2.79% of immunologically normal persons (43). Given that the 50% infective dose (ID₅₀) for C. hominis is less than 1/10 that of the IOWA isolate (44), ingestion of only one oocyst of a more infectious isolate may lead to a higher incidence of infection in the general immunocompetent population.

Cryptosporidiosis in industrialized nations is often associated with recreational water use (45, 46), animals on petting farms (47, 48), and day care centers (20, 49, 50). The small number of oocysts required to induce infection, susceptibility of young children, high risk of contamination of surfaces by diaper changing and inadequate hand washing, and the tendency of children to put nonfood items in their mouths make day care centers opportune sites for cryptosporidiosis outbreaks (51). While fecal-oral transmission is indisputably the major route of infection, transmission via coughing and fomites is also possible in situations of close contact (20).

Clinical Illness

While some infections go undetected, symptomatic cryptosporidiosis typically produces moderate to severe watery diarrhea, abdominal pain, vomiting, nausea, fever, anorexia, dehydration, and weight loss (39, 40). Host immune status dramatically alters the course of disease. Otherwise healthy individuals typically experience transient gastroenteritis lasting up to 2 weeks and recover without treatment (39). In contrast, persons with immunoglobulin deficiencies, renal failure, or inflammatory bowel disease, people receiving immunosuppressive treatment for other illnesses, and those with HIV/AIDS and not treated with ART often suffer fatal cryptosporidiosis following months of intractable diarrhea and wasting (40, 52). Immunocompromised individuals may experience disseminated cryptosporidiosis in other organ systems. In addition to the gastrointestinal and respiratory tracts, Cryptosporidium forms have been identified in the hepatobiliary system (53,–58), pancreas (56, 59), and urinary bladder (60), highlighting the unusual ability of this parasite to colonize most, if not all, mucosal epithelial surfaces. These widespread infections have been observed mostly in hosts with dysfunctional immune systems, which are unable to control or eliminate the parasite.

Although respiratory cryptosporidiosis in humans has been reported to be uncommon, it produces symptoms that are indistinguishable from those associated with other common respiratory illnesses. Upper respiratory cryptosporidiosis may cause inflammation of the nasal mucosa, sinuses, larynx, and trachea, accompanied by nasal discharge and voice change (54, 61, 62). Cryptosporidiosis of the lower respiratory tract typically results in productive cough, dyspnea, fever, and hypoxemia (63,–66).

Natural Infection

The first cases of cryptosporidiosis of the respiratory tract were reported for flocks of commercially grown turkeys with cough, nasal and ocular discharge, poor weight gain, and absence of gastrointestinal signs (67). Cryptosporidial forms attached to the ciliated epithelial border were identified on histologic sections of the trachea, bronchi, and nasal turbinates. Deciliation of infected cells was frequently noted. The mucosa was thickened, with hyperplastic epithelial cells, dilated mucous glands, and a mixed inflammatory infiltrate of the lamina propria (67). Cryptosporidiosis has also been identified in the sinuses (68) and conjunctiva (69, 70) of birds, and histologic changes remain remarkably similar across affected tissues, as well as across species. Respiratory tract cryptosporidiosis has been confirmed for turkeys (68, 71) and other commercially raised birds, including chickens (72,–74), partridges (75), peaco*cks (69), pheasants (76), and quail (77). Cases have also been documented for raptors (70, 78) and a jungle fowl (79). Cryptosporidium infections may occur in the respiratory tracts of avian species with or without gastrointestinal cryptosporidiosis. The paucity of other etiologic agents recovered from the respiratory tract and the frequent absence of gastrointestinal signs and pathology have helped to establish Cryptosporidium as a primary agent of avian respiratory disease that is capable of transmission via droplets and fomites (68).

Naturally occurring respiratory cryptosporidiosis is not limited to birds. Sporadic cases have been reported for a calf with confirmed cryptosporidial diarrhea (80) and several sheep from an abattoir (81). Four rhesus macaques, experimentally infected with simian immunodeficiency virus to simulate HIV infection in humans, subsequently developed disseminated cryptosporidiosis of the respiratory and gastrointestinal tracts and the bile and pancreatic ducts (82). In two of these primates, Cryptosporidium forms were found within alveoli (83), consistent with a well-established infection of the deep lung.

Experimental Infection

Respiratory cryptosporidiosis has been induced successfully in turkeys (84), chickens (85), and immunosuppressed rats (86, 87) by intratracheal inoculation, resulting in clinical signs and pathology mimicking those of natural infection. In turkeys, when C. baileyi oocysts were introduced directly into the trachea, respiratory signs and mortality ensued, while oral inoculation caused no clinical signs or loss of life (84). Pigs have also been explored as an animal model for respiratory cryptosporidiosis (88).

Preliminary findings from the gnotobiotic piglet model have shown that Cryptosporidium can readily be transmitted by inhalation. In our laboratory, five pairs of piglets were housed in sterile microbiological isolators. The pairs were physically separated but were allowed to share the same sterile air. They were fed from distinct food sources and were handled using separate sets of gloves. One piglet in each pair was intranasally inoculated with C. hominis oocysts. Consistent with serial infection, oocysts were shed first in the feces of each inoculated piglet, followed several days later by fecal shedding from each unchallenged piglet. Tracheal and pulmonary histologic sections taken 2 weeks after inoculation revealed no evidence of infection. Even if transient respiratory cryptosporidiosis was not substantiated, this work demonstrated that a patent intestinal infection with Cryptosporidium was established via airborne transmission between mammals (S. Tzipori, unpublished data).

Initial Descriptions

Cryptosporidium organisms in the respiratory tract of a human were first reported at autopsy in the trachea of a boy with congenital hypogammaglobulinemia and intestinal cryptosporidiosis (34) (Table 1). Cryptosporidium was subsequently identified at autopsy in the bronchial trees of two children with severe combined immunodeficiency (59, 89). Numerous oocysts were found in both endotracheal and fecal samples obtained from a newborn diagnosed with an immunoglobulin deficiency (90). Oocysts were found in sputum and maxillary sinus aspirates from an adolescent with congenital hypogammaglobulinemia and chronic intestinal cryptosporidiosis (54). More recently, a Turkish child with combined immunodeficiency harbored cryptosporidial oocysts in both tracheal and stool specimens (53). Each of these patients with congenital immune dysfunction manifested cryptosporidiosis in more than one organ system. In addition to intestinal and respiratory infections, two patients had evidence of hepatobiliary invasion (53, 54), and one had confirmed cryptosporidia in the pancreatic duct (59).

Respiratory cryptosporidiosis has also been reported for individuals with induced immunosuppression. A patient undergoing intensive treatment for leukemia developed both pulmonary and intestinal cryptosporidiosis, and eventually succumbed (91) (Table 1). In India, a child receiving corticosteroid treatment for nephrotic syndrome developed respiratory cryptosporidiosis with intestinal involvement (92). A woman with an unspecified immunodeficiency associated with lymphoma acquired cryptosporidiosis in the bronchial tree; though she did not have diarrhea, cryptosporidial forms within the duodenum and gallbladder were discovered at autopsy (55). A bone marrow transplant recipient developed respiratory cryptosporidiosis diagnosed by bronchoalveolar lavage (BAL). Interestingly, diarrhea was absent, fecal examination for oocysts was negative, and there was no histologic evidence of Cryptosporidium infection in either the respiratory or intestinal tract at autopsy (93). Because they lacked gastrointestinal symptoms and oocyst excretion, the latter cases establish the possibility of primary respiratory infection with Cryptosporidium, which may have been acquired by inhalation of expectorated droplets or by contact with fomites.

Advent of HIV

As recently as 30 years ago, human cryptosporidiosis was still considered an uncommon parasitic infection. Fewer than 50 cases were reported by 1983, with two-thirds diagnosed in AIDS patients (52). As the AIDS epidemic unfolded, cryptosporidiosis became a marker for identifying people with AIDS (94,–96). Predictably, as the human burden of AIDS mounted, so too did the incidence of Cryptosporidium infection in this subpopulation (97). HIV infection rapidly became a new risk factor for cryptosporidiosis, quickly outnumbering cases with other underlying causes.

In the absence of effective treatment for either Cryptosporidium or HIV infection, cryptosporidiosis, which typically causes self-limiting gastroenteritis, resulted in relentless diarrhea and wasting in individuals with HIV/AIDS. Cryptosporidiosis was most often limited to the gastrointestinal tract, but infections in other organ systems were identified. Respiratory infections involving the nasal mucosa (61), tracheobronchial tree (56, 60, 98,–100) (Fig. 1), and deep lung (101,–103) were reported (Table 1). While these early HIV/AIDS patients acquired disseminated cryptosporidiosis presumably originating in the intestinal tract, later cases lacking evidence of gastrointestinal involvement hinted at the possibility of respiratory transmission of cryptosporidiosis (66, 104,–106). Regardless of the route of infection, all of these critically ill patients experienced fulminant disease, failed to respond to existing therapies, and succumbed.

Antiretroviral Therapy

The introduction of combination ART enabled HIV-infected patients with access to these drugs to live longer (107), largely due to a decrease in frequency and severity of opportunistic infections, including cryptosporidiosis (108, 109). Flanigan et al. (110) found that individuals with CD4 counts of >180 cells/mm³ experienced only transient infection with Cryptosporidium, while those with fewer CD4 cells developed chronic, unremitting diarrhea. Additional studies demonstrated that combination ART including protease inhibitors dramatically resolved otherwise intractable cryptosporidial diarrhea in HIV-infected patients (23, 24, 111).

As ART substantially reduced the incidence of cryptosporidiosis in those with HIV, the number of reported cases of respiratory tract infections diminished drastically in the United States. Across Europe, respiratory cryptosporidiosis as a complication of HIV/AIDS has been reported sporadically in Denmark (105, 106), France (57, 63, 112), Italy (64, 66), Spain (58, 113), United Kingdom (114), and the former Czechoslovakia (60) (Table 1). Isolated cases have been documented in disparate countries, including Chile (104), Iran (65), and Japan (115).

Evidence of Respiratory Transmission

While Cryptosporidium infection of the respiratory tract has been well documented, particularly for immunocompromised individuals, disease transmission via this route has yet to be substantiated. The presence of various cryptosporidial forms lining the bronchial epithelium of lung sections suggests that the protozoan may be capable of propagating within the human respiratory tract in much the same way that it parasitizes the gastrointestinal epithelium (57, 98) (Fig. 2). Likewise, demonstration of intramacrophagic oocysts and extracellular invasive forms (sporozoites or merozoites) (57, 63, 90) (Fig. 3) in respiratory secretions implies an active life cycle within the respiratory tract, where transmission of oocysts may be facilitated by coughing or expectoration.

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FIG 3

Extracellular (black arrows) and intracellular (white arrow) cryptosporidial forms in BAL fluid (Giemsa stain). Magnification, ×1,250. (Reprinted from reference 63 with permission.)

Hints of a respiratory dimension to parasite transmission have been promulgated by a few early epidemiological studies, mostly involving children presumed to be immunocompetent. In Switzerland, children diagnosed with diarrhea due to Cryptosporidium were more likely to have respiratory symptoms than children with diarrhea from other sources, suggesting that respiratory infection may be common but transient in healthy individuals (116). In rural Brazil, 50% of children with intestinal cryptosporidiosis had unexplained respiratory signs (117). In Bangladesh, one-third of children under the age of 2 years and diagnosed with cryptosporidial diarrhea also had an unexplained cough (118). In Gaza, half of children with gastrointestinal cryptosporidiosis also had respiratory symptoms; interestingly, 10% of children with primary respiratory disease (in the absence of diarrhea) were also shedding Cryptosporidium in feces (119). The latter finding leads to speculation that the respiratory system may serve as a viable alternative to the gut for parasite propagation, transmission, and diagnosis, with or without apparent respiratory symptoms.

While zoonotic fecal-oral transmission of cryptosporidiosis is well documented, to our knowledge, zoonotic airborne transmission of cryptosporidiosis has been reported only once. A veterinarian who administered oral fluids to a calf with confirmed cryptosporidial diarrhea subsequently developed intestinal cryptosporidiosis herself after sniffing the contents of the stomach tube while it was positioned in the animal (120). Infection presumably occurred by inhalation of oocysts, though she exhibited only gastrointestinal symptoms, and no respiratory samples were taken to further characterize the extent of the infection in the respiratory tract. Oocyst inhalation has been proposed by others (59, 102). This possible mechanism of infection is supported by a few cases of respiratory cryptosporidiosis that developed in immunodeficient patients without diarrhea or evidence of fecal oocyst shedding (55, 66, 93, 105).

To our knowledge, prior to 2010, there were only two known cases of respiratory cryptosporidiosis in immunocompetent individuals. The first was in an infant in Papua New Guinea who suffered from laryngotracheitis and diarrhea due to Cryptosporidium (62) (Table 1). The second was in a young Spanish woman with no evidence of diarrhea, Cryptosporidium in stool, or HIV infection (121). In the latter case, respiratory cryptosporidiosis without an apparent gastrointestinal component again suggests possible transmission via inhalation.

Currently, the strongest evidence for person-to-person respiratory transmission of cryptosporidiosis in immunocompetent children is the work of Mor et al. (25). Their study demonstrated that one-third (35%) of Ugandan children diagnosed with intestinal cryptosporidiosis and cough had sputum samples containing Cryptosporidium DNA, suggesting the presence of organisms in the respiratory tract. No parasite DNA was detected in saliva from any of the children with positive sputum, indicating little possibility of sputum contamination by gastrointestinal contents. The identification of numerous children with Cryptosporidium DNA (and possibly organisms) in their respiratory secretions further hints at transmission between persons. In addition, of the 17 children with both stool and sputum samples positive for Cryptosporidium spp., 16 were seronegative for HIV and 10 were normally nourished (25). This finding suggests that respiratory cryptosporidiosis may occur commonly in immunocompetent individuals.

Other Possible Routes of Infection

Other possible routes of infection for respiratory cryptosporidiosis have been proposed. In patients with both intestinal and respiratory involvement, transient respiratory infection resulting from aspiration of gastrointestinal contents during vomiting has been suggested (59, 91, 101). While this pathogenesis is certainly plausible, at least one case demonstrated the presence of Cryptosporidium organisms in bronchial samples for over 3 months, reducing the likelihood of a fleeting respiratory infection precipitated by emesis (99). There is some evidence to support the spread of cryptosporidiosis from the intestinal tract to the respiratory system via circulation. Histopathology samples from one patient revealed parasite forms within the colonic vasculature, hinting that dissemination of the organism to the respiratory tree may have occurred through a hematogenous route (91). In vitro, Cryptosporidium organisms have successfully replicated in murine macrophages, which may help to explain both the spread of extraintestinal infections and the inability of immunosuppressed individuals to eradicate the parasite (122). In addition, early experimental studies in mice were successful in establishing patent gastrointestinal infections by intravenous inoculation of oocysts (123).

Since immunocompromised hosts are often concurrently infected with more than one pathogen, some have considered Cryptosporidium only a secondary invader of the respiratory tract. Numerous cases of respiratory cryptosporidiosis have been characterized by coinfections with pathogens, including cytomegalovirus (89, 93, 99), Pneumocystis jirovecii (105, 114), and Mycobacterium (58, 113). Mixed infections involving multiple pathogens have also been identified (59, 98, 100,–102, 112). Nonetheless, Cryptosporidium has been solely recovered from the respiratory tracts of a substantial number of patients with clinical disease (25, 58, 61, 62, 65, 66, 92, 104, 113, 115), suggesting a primary role for the organism as a respiratory pathogen. While two patients from whom only Cryptosporidium was recovered were asymptomatic and lacked radiographic evidence of lung disease (58), the overwhelming majority had a cough, with or without respiratory distress, and pulmonary infiltrates on thoracic radiographs. Furthermore, Cryptosporidium has been the only organism identified deep within the lung by BAL (57, 63, 64, 103, 105, 106, 121) and autopsy (60), implying an intimate association between the parasite and the respiratory tract.

Respiratory Samples

Respiratory samples obtained by sputum induction, tracheal aspiration, or BAL are examined for thick-walled Cryptosporidium oocysts and invasive forms of the parasite, including sporozoites and merozoites. Infective oocysts in respiratory secretions have been identified successfully by use of a variety of staining techniques, including acid-fast (57, 58, 113), auramine (58, 63, 100), and indirect immunofluorescence (58, 105, 106) staining. Sporozoites and merozoites have been recovered from BAL fluid specimens stained with Giemsa stain (57, 63) (Fig. 3), but these forms are reported less often, likely because their presence is not detectable by more widely used acid-fast and fluorescence staining methods. Ideally, both Giemsa and modified acid-fast or fluorescent stains would be utilized to identify all cryptosporidial forms present in respiratory specimens, which would more fully characterize the extent of infection and also reduce the likelihood of false-negative results. More recently, Mor et al. (25) successfully employed PCR-restriction fragment length polymorphism (PCR-RFLP) analysis to both diagnose infection and type Cryptosporidium species in respiratory samples.

Histopathology

Although this requires a more invasive evaluation, respiratory cryptosporidiosis may be diagnosed by detection of organisms in biopsy or autopsy specimens. Histopathologic sections stained with hematoxylin-eosin reveal multiple parasite forms lining the mucosal epithelium at the luminal surface of the trachea, bronchi, and bronchioles, with occasional organisms identified within bronchial mucous glands (60, 98, 102, 103) (Fig. 2). Bronchial and lung biopsy specimens have yielded intra- and extracellular cryptosporidia, including sporozoites or merozoites, as well as oocysts on the bronchoepithelial surface (57, 112).

Radiology

Radiographic findings are not pathognomonic for cryptosporidiosis, but evidence of pulmonary interstitial disease is often present (58, 63, 64, 66, 90, 112, 113).

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Jerlyn K. Sponseller, D.V.M., M.P.H., is an instructor in the Department of Infectious Disease and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, and in the Department of Public Health and Community Medicine, Tufts University School of Medicine, Boston, MA. She earned her veterinary degree at Purdue University, West Lafayette, IN, and completed her public health training at the University of Tennessee, Knoxville, TN. Dr. Sponseller is currently pursuing a Ph.D. by examining respiratory cryptosporidiosis and transmission in Ugandan children.

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Jeffrey K. Griffiths, M.D., M.P.H., and T.M., is Director of Global Health and Professor of Public Health and of Medicine in the Department of Public Health and Community Medicine, Tufts University School of Medicine, Boston, MA. He is also an Adjunct Professor at both Tufts Friedman School of Nutrition Science and Policy and Tufts School of Engineering. Dr. Griffiths has a major interest in infectious diseases, especially the human, animal, and environmental epidemiologies of Cryptosporidium. His other research interests include linkages between human health, nutrition, and the environment. These include the relationships between malnutrition, aflatoxins, heavy metals, air pollution, diarrhea, and pneumonia. Dr. Griffiths is currently involved in several projects focused in East Africa. He is the Program Director for the USAID Nutrition Innovation Lab for Collaborative Research in Africa and the Principal Investigator for an NIH-funded study of respiratory cryptosporidiosis and transmission in Uganda.

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Saul Tzipori, B.V.Sc., Ph.D., D.V.Sc., F.R.C.V.S., is Distinguished Professor and Chair of the Department of Infectious Disease and Global Health, Agnes Varis University Chair in Science and Society, and Director of the New England Regional Biosafety Laboratory at Tufts University Cummings School of Veterinary Medicine, North Grafton, MA, where he has been a faculty member since 1991. He has extensive research experience in infectious diseases of humans and domestic animals, with a particular interest in enteric pathogens (viruses, bacteria, fungi, and protozoa). His work includes investigating relative contributions of virulence attributes to pathogenesis and disease, screening and evaluation of chemical or immune-based therapeutic agents, and vaccine development. Dr. Tzipori is best known for his expertise on cryptosporidiosis, microsporidiosis, Escherichia coli O157:H7 diarrhea and hemolytic-uremic syndrome, shigellosis, and, more recently, Clostridium difficile. He is also the Tufts Principal Investigator of RESPOND, a USAID global health project within the Emerging Pandemic Threats program.

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