Serial Dilution Guide for Laboratory Sciences (2024)

Serial dilution and direct dilution both yield a range of concentrations. However, the key distinction lies in their methods:

Serial Dilution Series: Each concentration is created by diluting the previous one. This approach allows for accurate dilutions across a broader concentration range. For instance, if a stock solution in the first column is diluted 11 times (with a factor of 3 each time), the final column would be 311 = 177,147 times more dilute.

Direct Dilution Series: All concentrations are derived by taking varying amounts from a single stock solution. These can be more straightforward to set up, and negate the risk that an error affects all subsequent concentrations.

Serial Dilution Guide for Laboratory Sciences (2024)
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